流式实验常用Protocols
Protocols
Acquisition and Analysis
Apoptosis
Annexin V with Cell Surface Antibody Staining for Suspension Cells
Receptor-Mediated Apoptosis in Mouse Cells Using Anti-Fas/CD95 (Clone Jo2)
Receptor-Mediated Apoptosis in Human Cells Using Anti-Fas/CD95 (Clone DX2)
Cell Cycle/DNA Analysis
Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs
Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes
Staining Procedure for Flow Cytometric Detection of Human Cyclins
Cytokine (Intracellular Staining) Sample Preparation
Cell Fixation/Permeabilization Kits for Intracellular Cytokine Analysis
Generation of Human IL-17–Producing Cells (Th17-like Cells) in Vitro
Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis
General
Bulk Erythrocyte Lysing with Ammonium Chloride for Flow Cytometry Immunophenotyping
Cell Surface Staining of Stem Cell Lines and Other Adherent Cells for Flow Cytometry
Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs)
Human Sample Preparation
Cell Surface Staining of Human PBMCs and Suspension Cell Lines
Direct Immunofluorescence Staining of Whole Blood Using a Lyse/No-Wash Procedure
Direct Immunofluorescence Staining of Whole Blood Using a Lyse/Wash Procedure
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs
Mouse/Rat Sample Preparation
The Uses of BD Fc Block in Immunophenotyping of Mouse or Rat Leukocytes
Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes
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